The study was planned to investigate the occupational stress among railway employees working in Railways in Secunderabad Division. The study aimed to find out whether level of supervision, job tenure and department in which they are working have any significant influence on occupational stress. A sample of 480 employees was selected by systematic random sampling technique. Occupational Stress Inventory by Samuel H. Osipow and Spokane, A.R. (1987) was used for data collection. The data was analyzed by using statistical techniques, Mean, SD and ‘t’ test. The results reveal that there is significant difference between long job tenure and short job tenure employees in their occupational stress, whereas low and high supervisor employees and employees working in mechanical and commercial department have same level of occupational stress.