The study aimed at establishing barriers to the teaching and learning of comprehensive sexuality education in selected secondary schools in Lusaka province. The objective of the study was to: establish barriers to the teaching of comprehensive sexuality education in selected secondary schools in Lusaka Province. A descriptive survey was used in conducting this research. The study used both qualitative and quantitative methods of data collection. The sample consisted 172 respondents: 88 girls and 84 boys drawn from six secondary schools in Lusaka province. In selecting the participants, simple random sampling technique was used. The study employed the questionnaire and focus group discussion to ensure validity of the findings. The quantitative data was analysed using the statistical package for social sciences computer software while the qualitative data was analysed by coding and grouping the emerging themes. It emerged from the study that culture is one of the major barriers to the teaching of comprehensive sexuality education in secondary schools. This is evident by 70.9% of respondents who said that culture affect the teaching of comprehensive of sexuality education in schools. 52.3% of respondents also indicated that discussing issues of sexuality in public was considered to be inappropriate, this is as a result of the influence of their culture which restricts discussion of such matters in public. The study further revealed that 54.1% of pupils indicated that comprehensive sexuality education was not taught in a co-ordinated manner. Arising from the findings of the study, the following recommendations were made: 1. The Ministry of Education should supply more books on sexuality education. The Ministry of Education should make CSE a stand-alone subject rather than integrating it in other subjects