This study is an attempt to explore the influence of interaction effects of organizational climate, education and Gender on job satisfaction. Moreover it is also to study the effect of independent variables i.e. organizational climate, education and Gender on dependent variable i.e. Job satisfaction. In order to study, 180 subjects were taken including 90 males and 90 females with Professional and Traditional education from different public undertakings. F-test and New-man kaul test were also administered. The results show that organizational climate, education and Gender effect job satisfaction.